AHF NCI Collection
Analytical Investigations 781101 - 781130
Fields
- Type
- Bibliography
- Outline
- Rept, Other Report
- Outline
- Named Organization
- AHF, American Health Foundation
- Hewlett Packard
- Thermo Electron
- Hewlett Packard
- Characteristic
- Extra
- Author
- Brunnemann, K.D.
- Named Person
- Fink, W.
- Harvey, W. R.
Research Professional- Hofer
- Moser
- Oakley, E.
- Harvey, W. R.
Document Images
PME RESEARCH LABORATORY, November 1978
PROJECT TITLE ANALYTICAL INVESTIGATIONS
PERIOD COVERED~ . NOVEMBER list - NOVEMBER 30th
WRITTEN BY K.D. BRUNNEMANN
APPROVED BY W. FINK
I. TRAVELLING
October 26-27 : HPLC course at Hewlett-Packard, Karlsruhe-
Waldbronn (Germany) on the operation of the HP1084/A/B
liquid chromatog,raph. Excellent course.
October 30- November 1 : Tobacco Chemists' Research Confer-
ence, Montreal (Canada).
November 2 : Visit at Thermo-Electron Corp., manufacturer
of the nitrosamine detector TEA. Discussed trouble-shooting
of the TEA and their current research programme.
November 3 - November 6 : Visit at the Naylor Dana Institute,
Valhalla, N.Y.
November 7 - November 10 : Visit at Philip~Morris USA,
Richmon&.
II. ANALYSISOF NNN IN NINO No:. 39
The current NINO run is being,analyzed during all phases of
the process for the presence of both volatile (Mr. Moser's
group) and non-volatile nitrosamines. The analytical scheme
has been worked out for NNN and the equipment (Rheodyne valve
Loop injector and Tracor liquid pump), has beemreceived. A
new lab technician (Mr. Hofer) started work on November 13th.
Results of the NNN analyses will be presented in detail in
the next monthly report. It is also p1'anned to combine the
work-up for both volatile an6non-volatile nitrosamines in
one step.
III.ANALYSISOF SORBIC ACID IN TOBACCO AND/OR TOBACCO EXTRACTS
A review of the literature and personali discussions with
E. Oakley and W.R. Harvey in Richmond were undertaken.
There are two basic methods : steam distillation and
direct UV determination at 220 nm (1) andla colorimetric
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PME RESEARCH LABORATORY, November 1978
method oxidizing sorbic acid'to malonaldehyde which
reacts with thiobarbituri~c acid to form a red pigment;
h max = 532 nm, logf = 5.2 (2-5).
It is planned to develop an HPLC method for sorbic acid.
A recent publication (6)1 reports on the inhibition of
nitrosamine formation in vitro by sorbic acid. Appli-
cation of this phenomenon to our NINO process should be
considered.
REFERENCES
(1) Philip Morris Method No. E-29
(2) Philip Morris Method No. E-29A
(3) H~. Schmidt, Z. Anal. Chem. 178, 173-1184 (11960)
(4) F.S. Nury and H.R. Bolin, J. Food Sci.27, 370-372 (1972)
(5) W.R. Harvey et al, Tob. Sci. 19, 133-135 (1975)
(6) K. Tanaka et al, Fd. Cosmet. Toxicoll. 16, 209-215 (1978)
K.D. Brunnemann
J
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