AHF NCI Collection
Chemical Studies on Tobacco Smoke Lxvi. Assessment of Volatile and Tobacco-Specific N-Nitrosamines in the Smoke of Some Selected West German Cigarettes
Fields
- Type
- Bibliography
- Chart/Graph
- Scrt, Scientific Report
- Chart/Graph
- Named Organization
- NCI, Natl Cancer Inst
- Rheodyne
- Thermal Electron
- Waters Associates
- FDA, Food and Drug Administration
- Federal Ministry for Youth Family&
- Rheodyne
- Author
- Adams, J.D.
- Hoffmann, D.
- Ruhl, C.
- Hoffmann, D.
- Named Person
- Borgwald, H.
- Fazio, T.
Document Images
Ghrh ..o-an.
r nonu~n.
Cu~umnN~wn.
6S p AltQ1.
M.IIID
/ 6CE
, 7tlER-,I EMEpi.
\ IJ14tYZE0.
Fig. 1 Analytical procedtrre for the determination
of volatile nitrosamines in cigarette smoke.
S
60 CIGARETTES SMOKED1NTq
CITRATE BUFFER(pH'4.5)
CONT. 20mM ASCORBIC ACID
I.NNN-14C
2.EXTRACT WITH CH2CI2
AQUEOUS
LAYER
2.
~
ELUTE WITH 4:1
CH2C12: DMK
~
CHZC12
EXTRACT
I.DRY,Nh2SO4
2. CONCENTRATE
3. COLUMN CHROM..
90g A1203
ACT. II - I II'
I.
ELUTE WITH
CH2CI2
(VOLATILE NITROSAMINES),
(NON-VOLATILE NITROSAMINES)
Fig. 2 Analytical scheme for the determination of
nonvolatile nitrosmaines in cigarette smoke.
0 To TRaPS
Qd GIa IMTnKE
Q TO SM[NfINGM.ACHINE
QCOOUtiG WATER iN.
Fig. 3. Apparatus used to collect sidestream smoke generated
by a cigarette.
S
0
+
8
---F---
16
TIME DMINI)
a
z
i -
24 32
Fi~g. 4 Gas-liquid chromatogram of volatile
nitrosamines from mainstream smoke
of a German cigarette.
~
NNN
w
Ef)
z
0
a
U)
w
Q~:
NAfB
I
NNK
UL
24 36
12
TIME (MIN.)
Fig. 5 High-performance liquid chromatogram of
nonvolatile nitrosamines from mainstream
smoke of a German cigarette.
